Today´s world is reflected in words such as globalization, economic crisis, market economy and competitiveness. All these influence business entities. An enterprise that does not keep pace with current trends, innovates, seek improvements in quality or assess the effectiveness of its activities, is at risk of stagnating. Similar trends also prevail in logistics enterprises in Slovakia. The aim of this study was to analyse organizational culture in logistics enterprises because it is one of the key factors affecting the quality of the products and the overall competitiveness of an enterprise. Utilizing the Organizational Culture Assessment Instrument (OCAI) we diagnosed the dominant trend within Slovak logistics enterprises. On the whole these enterprises have a hierarchical organizational culture. However, employees are of the opinion that there should be a more familial work environment, typical of a clan organizational culture. Whilst enterprises continue to underestimate this factor, they will miss an important tool to achieve better success rates in a tough competitive environment.